Literature summary for 1.14.15.6 extracted from

Schwarz, D.; Kisselev, P.; Wessel, R.; Jueptner, O.; Schmid, R.D.
alpha-Branched 1,2-diacyl phosphatidylcholines as effectors of activity of cytochrome P450SCC (CYP11A1). Modeling the structure of the fatty acyl chain region of cardiolipin (1996), J. Biol. Chem., 271, 12840-12846.

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Activating Compound

Activating Compound	Comment	Organism	Structure
1,2-di-(2'-hexyl-decanoyl)-sn-glycero-3-phosphocholine	alpha-branched phosphatidylcholine, inclusion in vesicle-reconstituted system, partially in connection with the nonactivator lipids dimyristoyl-/dioleoyl-phosphatidylcholine, efficiency close to cardiolipin	Bos taurus
1,2-di-(2'-octyl-dodecanoyl)-sn-glycero-phosphocholine	alpha-branched phosphatidylcholine, inclusion in vesicle-reconstituted system, partially in connection with the nonactivator lipids dimyristoyl-/dioleoyl-phosphatidylcholine, efficiency close to cardiolipin	Bos taurus
cardiolipin	most potent and effective activator lipid, binds to enzyme and enhances the binding of cholesterol	Bos taurus
additional information	head and fatty acid groups have large effects on stimulation of activity, head group appears as major determinant of the lipid interaction with the P-450scc, 1-2 and 3-4 molecules of cardiolipin and the branched phosphatidylcholines, respectively, bind highly specific to P-450scc at an effector site distant from cholesterol binding site with a resultant stabilization of an optimal cholesterol-binding conformation of P-450scc	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial inner membrane	inner membrane	Bos taurus	5743	-
mitochondrial inner membrane	matrix side	Bos taurus	5743	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2	Bos taurus	-	pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O	precursor of all steroid hormones	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal cortex	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2	-	Bos taurus	pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O	precursor of all steroid hormones	?
cholesterol + reduced adrenodoxin + O2	-	Bos taurus	pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bos taurus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	42	tested in different dimyristoyl-phosphatidylcholine vesicles, breaks in activity at 27-30°C	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
adrenodoxin	-	Bos taurus
heme	-	Bos taurus
NADPH	NADPH-linked adrenodoxin reductase	Bos taurus

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Release 2023.1 (January 2023)