Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | integral to membrane | Bos taurus | 5743 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | several cations and anions promote the dissociation of adrenodoxin-cytochrome P-450scc complex, decreasing order: K+, Cs+, Rb+, Na+, Li+ and SCN-, Br-, Cl-, respectively. The order for dissotiation of adrenodoxin-adrenodoxin reductase complex: Rb+, Cs+, K+, Li+, Na+ | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | intermediates (22R)-22-hydroxycholesterol and (20R,22R)-20,22-dihydroxycholesterol from hydroxylation | Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | regioselective and stereospecific transformation | Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | intermediates 22R-hydroxycholesterol and 20alpha,22R-dihydroxycholesterol from hydroxylation | Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
additional information | electron shuttle between flavoprotein and cytochrome, biphasic reduction kinetics, rapid association of cytochrome and adrenodoxin, slower intracomplex electron transfer from iron-sulfur center of adrenodoxin to heme of cytochrome P-450scc | Bos taurus | ? | - |
? | |
additional information | suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex | Bos taurus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bos taurus |
7 | - |
reduction of P-450scc-cholesterol complex by reduced adrenodoxin | Bos taurus |
7.2 | - |
maximal adrenodoxin-induced absorbance with 0.03 mM cholesterol | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
heme | - |
Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |