Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | oxidized form, high affinity to P-450scc, inhibits side chain cleavage by competition with reduced form | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
reduced adrenodoxin | free | Bos taurus | |
0.00056 | - |
reduced adrenodoxin | +/-0.00004, complex between adrenodoxin and adrenodoxin reductase | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | integral to membrane | Bos taurus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 + H+ | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
r | |
additional information | suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex | Bos taurus | ? | - |
? | |
additional information | reduction of adrenodoxin must be rate-limiting step | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | transports electrons from adrenodoxin reductase to cytochrome P-450 by shuttling | Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |