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Literature summary for 1.14.15.4 extracted from
- Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level (2012), J. Steroid Biochem. Mol. Biol., 132, 57-65.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His6-tagged CYP11B2 mature form without the mitochondrial targeting sequence of 24 residues at the N-terminus in Escherichia coli, co-expression with the molecular chaperones GroEL and GroES | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ketoconazole | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, recombinant His-tagged enzyme without the mitochondrial targeting sequence, SDS-PAGE, x * 55890, sequence calculation of enzyme without the mitochondrial targeting sequence but with His6-tag | Homo sapiens |
| 55890 | - |
x * 50000, recombinant His-tagged enzyme without the mitochondrial targeting sequence, SDS-PAGE, x * 55890, sequence calculation of enzyme without the mitochondrial targeting sequence but with His6-tag | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11-deoxycorticosterone + reduced adrenodoxin + O2 | Homo sapiens | - |
corticosterone + oxidized adrenodoxin + H2O | - |
? | |
| 18-hydroxycorticosterone + reduced adrenodoxin + O2 | Homo sapiens | - |
aldosterone + oxidized adrenodoxin + H2O | - |
? | |
| corticosterone + reduced adrenodoxin + O2 | Homo sapiens | - |
18-hydroxycorticosterone + oxidized adrenodoxin + H2O | - |
? | |
| additional information | Homo sapiens | bifunctional enzyme catalyzing the biosynthesis of aldosterone by successive 11beta- and 18-hydroxylation followed by an 18-oxidation of 11-deoxycorticosterone | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P19099 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged CYP11B2 from Escherichia coli by nickel affinity chromatography and anion and cation exchange chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11-deoxycorticosterone + reduced adrenodoxin + O2 | - |
Homo sapiens | corticosterone + oxidized adrenodoxin + H2O | - |
? | |
| 18-hydroxycorticosterone + reduced adrenodoxin + O2 | - |
Homo sapiens | aldosterone + oxidized adrenodoxin + H2O | - |
? | |
| corticosterone + reduced adrenodoxin + O2 | - |
Homo sapiens | 18-hydroxycorticosterone + oxidized adrenodoxin + H2O | - |
? | |
| additional information | bifunctional enzyme catalyzing the biosynthesis of aldosterone by successive 11beta- and 18-hydroxylation followed by an 18-oxidation of 11-deoxycorticosterone | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant His-tagged enzyme without the mitochondrial targeting sequence, SDS-PAGE, x * 55890, sequence calculation of enzyme without the mitochondrial targeting sequence but with His6-tag | Homo sapiens |
| More | homology modeling and molecular docking of CYP11B2, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aldosterone synthase | - |
Homo sapiens |
| CYP11B2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adrenodoxin | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | human aldosterone synthase is responsible for the biosynthesis of aldosterone from 11-deoxycorticosterone in a sequential three-step reaction with two intermediates, corticosterone and 18-hydroxycorticosterone | Homo sapiens |
| additional information | homology modeling and molecular docking of CYP11B2, overview | Homo sapiens |
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Release 2023.1 (January 2023)
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