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Literature summary for 1.14.15.3 extracted from

  • Ramu, R.; Chang, C.; Chou, H.; Wu, L.; Chiang, C.; Yu, S.
    Regio-selective hydroxylation of gem-difluorinated octanes by alkane hydroxylase (AlkB) (2011), Tetrahedron Lett., 52, 2950-2953.
No PubMed abstract available
Search for more literature for 1.14.15.3

Application

Application Comment Organism
additional information the enzyme is useful in a whole-cell biotransformation system by recombinant expression in Escherichia coli to regioselectively synthesize 2,2-, 3,3- and 4,4-difluorooctan-1-ols, from simple and inexpensive starting materials, method evaluation, overview Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information development of a whole-cell biotransformation system expressing AlkB in Escherichia coli to regioselectively synthesize 2,2-, 3,3- and 4,4-difluorooctan-1-ols, from simple and inexpensive starting materials, method evaluation, overview Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane-bound protein Pseudomonas putida 16020
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a nonheme diiron monooxygenase Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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Pseudomonas putida GPo1 / ATCC29347
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information AlkB, a nonheme diiron monooxygenase, performs regioselectibe hydroxylation of gem-difluorinated octanes, synthesis of 2,2-, 3,3- and 4,4-difluorooctan-1-ols from 1-octanal and 2-, 3-, 4-octanones, NMR and GC/MS product analysis, overview. Reactions of AlkB with 3,3- and 4,4-difluorooctanes and 1,1,1-trifluoroctane, synthesis of 2,2-, 3,3- 4,4-difluorooctan-1-ols, and of 8,8-, 7,7-, 6,6- and 5,5-difluorooctan-1-ols, overview Pseudomonas putida ?
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 ?
additional information AlkB, a nonheme diiron monooxygenase, performs regioselectibe hydroxylation of gem-difluorinated octanes, synthesis of 2,2-, 3,3- and 4,4-difluorooctan-1-ols from 1-octanal and 2-, 3-, 4-octanones, NMR and GC/MS product analysis, overview. Reactions of AlkB with 3,3- and 4,4-difluorooctanes and 1,1,1-trifluoroctane, synthesis of 2,2-, 3,3- 4,4-difluorooctan-1-ols, and of 8,8-, 7,7-, 6,6- and 5,5-difluorooctan-1-ols, overview Pseudomonas putida GPo1 / ATCC29347 ?
-
 ?

Synonyms

Synonyms Comment Organism
alkane hydroxylase
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 Pseudomonas putida
AlkB
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 Pseudomonas putida
diiron omega-hydroxylase
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 Pseudomonas putida

General Information

General Information Comment Organism
additional information AlkB contains a nonheme diiron center as a catalytic site Pseudomonas putida