Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | reversible inhibitor | Pseudomonas oleovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain | Pseudomonas oleovorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 1 atom Fe per 42000 g/mol peptide | Pseudomonas oleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
n-alkane + NAD(P)H + H+ + O2 | Pseudomonas oleovorans | - |
n-alkanol + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
ferrous ions restore activity after treatment with EDTA and dithionite | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
- |
Pseudomonas oleovorans |
Storage Stability | Organism |
---|---|
-20°C, 1 month, under 50% activity retained | Pseudomonas oleovorans |
-70°C, 12 days, over 90% activity retained | Pseudomonas oleovorans |
-70°C, 6 months, over 50% activity retained | Pseudomonas oleovorans |
4°C, 5 days, under 50% activity retained | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,7-octadiene + NADH + H+ + O2 | epoxidation of simple, aliphatic terminal olefins | Pseudomonas oleovorans | 1,2-epoxy-7-octene + NAD+ + H2O | - |
? | |
2,5-dimethylhexane + NADH + H+ + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
cyclohexane + NADH + H+ + O2 | - |
Pseudomonas oleovorans | cyclohexanol + NAD+ + H2O | - |
? | |
fatty acid + NAD(P)H + H+ + O2 | chain length C6-C14 | Pseudomonas oleovorans | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
methylcyclohexane + NADH + H+ + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | - |
Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | rubredoxin is electron carrier | Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | chain length C6-C14 | Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay conditions | Pseudomonas oleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay conditions | Pseudomonas oleovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | hydroxylation needs also a NADH-dependent bacterial reductase | Pseudomonas oleovorans |