Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces griseolus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00054 | - |
calciol | vitamin D3 25-hydroxylation, pH 7.4, 30°C | Streptomyces griseolus | |
0.00059 | - |
vitamin D2 | vitamin D2 25-hydroxylation, pH 7.4, 30°C | Streptomyces griseolus | |
0.00091 | - |
calcidiol | 25-hydroxyvitamin D3 1alpha-hydroxylation, pH 7.4, 30°C | Streptomyces griseolus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseolus | P18326 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Streptomyces griseolus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Streptomyces griseolus | calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
calciol + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Streptomyces griseolus | calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
vitamin D2 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Streptomyces griseolus | 25-hydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP105A1 | - |
Streptomyces griseolus |
cytochrome P450SU-1 | - |
Streptomyces griseolus |
vitamin D3 dihydroxylase | - |
Streptomyces griseolus |