Application | Comment | Organism |
---|---|---|
pharmacology | modification at the C-1 position of a steroid is of pharmaceutical interest. Biotransformation can overcome tedious multistep chemical synthesis | Sorangium cellulosum |
Cloned (Comment) | Organism |
---|---|
expression in a soluble form in Escherichia coli | Sorangium cellulosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
androstenedione | pH and temperature not specified in the publication | Sorangium cellulosum | |
0.04 | - |
testosterone | pH and temperature not specified in the publication | Sorangium cellulosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50220 | - |
ESI-TOF mass spectrometry, holoenzyme | Sorangium cellulosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorangium cellulosum | A9FDB7 | Soce56 | - |
Purification (Comment) | Organism |
---|---|
- |
Sorangium cellulosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-oxoandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxy-11-oxoandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxyandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Escherichia coli-based whole-cell biocatalyst system | Sorangium cellulosum | 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster | Escherichia coli-based whole-cell biocatalyst system produces a mixture (1:2.5:3) of an unknown compound and 1alpha- and 9alpha-hydroxytestosterone acetate | ? |
Synonyms | Comment | Organism |
---|---|---|
CYP260A1 | - |
Sorangium cellulosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | cytochrome P-450-dependent monooxygenase | Sorangium cellulosum |