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Literature summary for 1.14.15.19 extracted from

  • Khatri, Y.; Ringle, M.; Lisurek, M.; von Kries, J.P.; Zapp, J.; Bernhardt, R.
    Substrate hunting for the myxobacterial CYP260A1 revealed new 1alpha-hydroxylated products from C-19 steroids (2016), Chembiochem, 17, 90-101.
    View publication on PubMed

Application

Application Comment Organism
pharmacology modification at the C-1 position of a steroid is of pharmaceutical interest. Biotransformation can overcome tedious multistep chemical synthesis Sorangium cellulosum

Cloned(Commentary)

Cloned (Comment) Organism
expression in a soluble form in Escherichia coli Sorangium cellulosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
androstenedione pH and temperature not specified in the publication Sorangium cellulosum
0.04
-
testosterone pH and temperature not specified in the publication Sorangium cellulosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50220
-
ESI-TOF mass spectrometry, holoenzyme Sorangium cellulosum

Organism

Organism UniProt Comment Textmining
Sorangium cellulosum A9FDB7 Soce56
-

Purification (Commentary)

Purification (Comment) Organism
-
Sorangium cellulosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-oxoandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Escherichia coli-based whole-cell biocatalyst system Sorangium cellulosum 1alpha-hydroxy-11-oxoandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Escherichia coli-based whole-cell biocatalyst system Sorangium cellulosum 1alpha-hydroxyandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Escherichia coli-based whole-cell biocatalyst system Sorangium cellulosum 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Escherichia coli-based whole-cell biocatalyst system Sorangium cellulosum 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster Escherichia coli-based whole-cell biocatalyst system produces a mixture (1:2.5:3) of an unknown compound and 1alpha- and 9alpha-hydroxytestosterone acetate ?

Synonyms

Synonyms Comment Organism
CYP260A1
-
Sorangium cellulosum

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 cytochrome P-450-dependent monooxygenase Sorangium cellulosum