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Literature summary for 1.14.15.18 extracted from

  • Yasutake, Y.; Fujii, Y.; Cheon, W.K.; Arisawa, A.; Tamura, T.
    Crystallization and preliminary X-ray diffraction studies of vitamin D3 hydroxylase, a novel cytochrome P450 isolated from Pseudonocardia autotrophica (2009), Acta Crystallogr. Sect. F, 65, 372-375.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
His-tag, expressed in Rhodococcus erythropolis and Escherichia coli Pseudonocardia autotrophica

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudonocardia autotrophica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45400
-
 x * 45400 Pseudonocardia autotrophica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
25-hydroxyvitamin D3 + NADPH + O2 Pseudonocardia autotrophica
-
 1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
 ?
cholecalciferol + NADPH + O2 Pseudonocardia autotrophica
-
 25-hydroxyvitamin D3 + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudonocardia autotrophica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudonocardia autotrophica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
25-hydroxyvitamin D3 + NADPH + O2
-
 Pseudonocardia autotrophica 1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
 ?
cholecalciferol + NADPH + O2
-
 Pseudonocardia autotrophica 25-hydroxyvitamin D3 + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 45400 Pseudonocardia autotrophica

Synonyms

Synonyms Comment Organism
vitamin D3 hydroxylase
-
 Pseudonocardia autotrophica

Cofactor

Cofactor Comment Organism Structure
cytochrome-P450
-
 Pseudonocardia autotrophica