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Literature summary for 1.14.15.18 extracted from

  • Sakaki, T.; Sawada, N.; Takeyama, K.i.; Kato, S.; Inouye, K.
    Enzymic properties of mouse 25-hydroxyvitamin D3 1alpha-hydroxylase expressed in Escherichia coli (1999), Eur. J. Biochem., 259, 731-738.
    View publication on PubMed
Search for more literature for 1.14.15.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
CO
-
 Mus musculus
ketoconazole
-
 Mus musculus
Metyrapone
-
 Mus musculus
vitamin D3
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 24,25-dihydroxycholecalciferol
-
 Mus musculus
0.0027
-
 25-hydroxycholecalciferol
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
25-hydroxycholecalciferol + NADPH + O2 Mus musculus recombinant in E. coli calcitriol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O cytochrome P-450 enzyme Mus musculus
a
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000002
-
 substrate 23,25-dihydroxycholecalciferol Mus musculus
0.0000011
-
 substrate 24-oxo-25-hydroxycholecalciferol Mus musculus
0.0000015
-
-
 Mus musculus
0.0000043
-
 substrate 24,25-dihydroxycholecalciferol Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(24R),25-dihydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
(24R),25-dihydroxycholecalciferol + NADPH + O2 higher activity than with 25-hydroxycholecalciferol Mus musculus 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
23,25-dihydroxycholecalciferol + NADPH + O2 low activity Mus musculus ?
-
 ?
23,25-dihydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus ?
-
 ?
24,25-dihydroxycholecalciferol + reduced adrenodoxin + O2
-
 Mus musculus 1alpha,24,25-trihydroxycholecalciferol + oxidized adrenodoxin + H2O
-
 r
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2 low activity Mus musculus ?
-
 ?
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus ?
-
 ?
24-oxo-25-hydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus ?
-
 ?
25-hydroxycholecalciferol + NADPH + O2 recombinant in E. coli Mus musculus calcitriol + NADP+ + H2O
-
 ?
calcidiol + NADPH + O2 recombinant enzyme Mus musculus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Mus musculus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
additional information the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity Mus musculus ?
-
 ?
additional information no activity with 24,25,26,27-tetranor-23-hydroxyvitamin D3 and vitamin D3 Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
CYB27B1
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mus musculus