Cloned (Comment) | Organism |
---|---|
expression in COS cells, reconstituted with recombinant cytochrome P-450, recombinant adrenodoxin, and recombinant adrenodoxin reductase | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction and expression in COS cell line of a chimeric fusion protein: vitaminD receptor ligand-binding domain with the yeast GAL4 DNA-binding domain, also expression of lacZ containing reporter plasmid | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,25-Dihydroxycholecalciferol | calcitriol; in vivo only inhibitory in vitamin D receptor containing cells, therefore: negative feedback inhibition is mediated by the enzyme through liganded vitamin D receptor | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE after in vitro-translation | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O35084 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | 25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase | Mus musculus | |
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | amino acid sequence determination | Mus musculus | |
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Mus musculus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |