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Literature summary for 1.14.15.18 extracted from

  • Gray, R.W.; Ghazarian, J.G.
    Solubilization and reconstitution of kidney 25-hydroxyvitamin D3 1 alpha- and 24-hydroxylases from vitamin D-replete pigs (1989), Biochem. J., 259, 561-568.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00097
-
25-hydroxycholecalciferol reconstituted system Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
ferredoxin, ferredoxin reductase and cytochrome P-450 Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of 1alpha-hydroxylating cytochrome P-450 Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000072
-
purified cytochrome P-450 component of 1alpha-hydroxylase, reconstituted system Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
25-hydroxycholecalciferol + NADPH + O2
-
Sus scrofa calcitriol + NADP+ + H2O
-
?
calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Sus scrofa calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Sus scrofa