Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
gene PaO, functional recombinant expression in Escherichia coli strain C43 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
0.006 | - |
pheophorbide a | pH 7.0, 24°C, recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast envelope | PaO is localized in the chloroplast inner envelope | Arabidopsis thaliana | 9941 | - |
gerontoplast | the enzyme contains a chloroplast transit peptide | Arabidopsis thaliana | 34400 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | Rieske-type iron-sulfur cluster-containing protein | Arabidopsis thaliana | |
Iron-sulfur cluster | AtPaO is a Rieske-type ironsulfur cluster-containing enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | PaO expression is correlated positively with senescence. The in vivo function of PaO is the degradation of pheide a during senescence | ? | - |
? | |
pheophorbide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Arabidopsis thaliana | - |
red chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9FYC2 | gene PaO | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
additional information | PaO activity is found only during senescence | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PaO expression is correlated positively with senescence. The in vivo function of PaO is the degradation of pheide a during senescence | Arabidopsis thaliana | ? | - |
? | |
pheophorbide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Arabidopsis thaliana | red chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACD1 | - |
Arabidopsis thaliana |
AtPaO | - |
Arabidopsis thaliana |
PAO | - |
Arabidopsis thaliana |
pheophorbide a oxygenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | AtPaO is a Rieske-type ironsulfur cluster-containing enzyme that is identical to Arabidopsis accelerated cell death 1 and homologous to lethal leaf spot 1 (LLS1) of maize | Arabidopsis thaliana |
metabolism | enzyme PaO seems to be a key regulator of chlorophyll catabolism | Arabidopsis thaliana |
physiological function | chlorophyll breakdown during senescence is an integral part of plant development and leads to the accumulation of colorless catabolites. The loss of green pigment is due to an oxygenolytic opening of the porphyrin macrocycle of pheophorbide (pheide) a followed by a reduction to yield a fluorescent chlorophyll catabolite. This step is comprised of the interaction of two enzymes, pheide a oxygenase (PaO) and red chl catabolite reductase. Senescence-related activity of PaO | Arabidopsis thaliana |