Literature summary for 1.14.15.16 extracted from

Beckman, M.J.; Tadikonda, P.; Werner, E.; Prahl, J.; Yamada, S.; DeLuca, H.F.
Human 25-hydroxyvitamin D3-24-hydroxylase, a multicatalytic enzyme (1996), Biochemistry, 35, 8465-8472.

Search for more literature for 1.14.15.16

Activating Compound

Activating Compound	Comment	Organism	Structure
adrenodoxin	required for activity of the recombinant enzyme	Homo sapiens
adrenodoxin reductase	required for activity of the recombinant enzyme	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf21 insect cells using the previously cloned cDNA in baculovirus (AcNPV-P450cc24), solubilized mitochondrial protein from Sf21 cells infected with recombinant virus AcNPV-P450cc24 cDNA express 24-hydroxylase activity when reconstituted with bovine adrenodoxin, adrenodoxin reductase, and NADPH	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
25-hydroxyvitamin D3 + NADPH + H+ + O2	-	Homo sapiens	24-oxo-25-hydroxyvitamin D3 + 23(S),25-dihydroxyvitamin D3 + 24(R),25-dihydroxyvitamin D3 + 24-oxo-23(S),25-dihydoxyvitamin D3 + NADP+ + H2O	-	?
25-hydroxyvitamin D3 + NADPH + H+ + O2	-	Homo sapiens	24,25,26,27-tetranor-23-hydroxyvitamin-D3 + NADP+ + H2O	-	?
calcidiol + NADPH + H+ + O2	-	Homo sapiens	secalciferol + NADP+ + H2O	-	?
calcitriol + NADPH + H+ + O2	the h-P450cc24 activity is approximately 10fold higher for calcitriol than for calcidiol	Homo sapiens	calcitetrol + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
25-hydroxyvitamin D3-24-hydroxylase	-	Homo sapiens
P450cc24	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	required for activity of the recombinant enzyme	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	P450cc24 is a multicatalytic enzyme catalyzing most, if not all, of the reactions in the C-24/C-23 pathway of 25-hydroxyvitamin D3 (calcidiol) metabolism	Homo sapiens

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Release 2023.1 (January 2023)