Literature summary for 1.14.15.15 extracted from

Fedorova, O.V.; Zernetkina, V.I.; Shilova, V.Y.; Grigorova, Y.N.; Juhasz, O.; Wei, W.; Marshall, C.A.; Lakatta, E.G.; Bagrov, A.Y.
Synthesis of an endogenous steroidal Na pump inhibitor marinobufagenin, implicated in human cardiovascular diseases, is initiated by CYP27A1 via bile acid pathway (2015), Circ. Cardiovasc. Genet., 8, 736-745.

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Application

Application	Comment	Organism
medicine	the endogenous steroidal Na/K-ATPase inhibitor, marinobufagenin, is synthesized in mammalian placenta and adrenal cortex from cholesterol through the novel acidic bile acid pathway, role of marinobufagenin in highly prevalent human cardiovascular diseases	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene CYP27A1, quantitative real-time PCR enzyme expression analysis	Homo sapiens
gene CYP27A1, quantitative real-time PCR enzyme expression analysis	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	silencing of gene CYP27A1 by targeting siRNA	Homo sapiens
additional information	silencing of gene CYP27A1 by targeting siRNA	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-
mitochondrion	-	Rattus norvegicus	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q02318	gene CYP27A1	-
Rattus norvegicus	P17178	gene CYP27A1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal gland	-	Homo sapiens	-
adrenal gland	-	Rattus norvegicus	-
adrenocortical cell	-	Homo sapiens	-
adrenocortical cell	-	Rattus norvegicus	-
JEG-3 cell	-	Homo sapiens	-
liver	-	Homo sapiens	-
liver	-	Rattus norvegicus	-
placenta	-	Homo sapiens	-
placenta	-	Rattus norvegicus	-
trophoblast	-	Homo sapiens	-
trophoblast	-	Rattus norvegicus	-

Synonyms

Synonyms	Comment	Organism
CYP27A1	-	Homo sapiens
CYP27A1	-	Rattus norvegicus

Expression

Organism	Comment	Expression
Rattus norvegicus	in vivo, in a high-salt administration experiment, male and female Dahl salt-sensitive rats become hypertensive after 4 weeks on a high-NaCl diet, their plasma marinobufagenin levels double, and adrenocortical CYP27A1 mRNA and protein increase 1.6fold and 2.0fold, respectively	up

General Information

General Information	Comment	Organism
malfunction	post-transcriptional silencing of the CYP27A1 gene in human trophoblast reduces the expression of CYP27A1 mRNA by 70%, reduces total bile acids by 2fold, and marinobufagenin levels by 67% when compared with nontreated cells or cells transfected with nontargeting siRNA	Homo sapiens
malfunction	post-transcriptional silencing of the CYP27A1 gene in rat adrenocortical cells reduces the expression of CYP27A1 mRNA by 70%, reduces total bile acids by 2fold, and marinobufagenin levels by 67% when compared with nontreated cells or cells transfected with nontargeting siRNA	Rattus norvegicus
metabolism	the enzyme initiates the biosynthesis of bile acids, pathway overview	Homo sapiens
metabolism	the enzyme initiates the biosynthesis of bile acids, pathway overview	Rattus norvegicus
physiological function	bioactive steroid marinobufagenin, an endogenous Na/K-ATPase bufadienolide inhibitor that is synthesized by adrenocortical and placental cells, is derived from cholesterol through the traditional steroidogenesis pathway initiated by enzyme CYP11A1, and via the acidic bile acid pathway, which is controlled by enzyme CYP27A1	Homo sapiens
physiological function	bioactive steroid marinobufagenin, an endogenous Na/K-ATPase bufadienolide inhibitor that is synthesized by adrenocortical and placental cells, is derived from cholesterol through the traditional steroidogenesis pathway initiated by enzyme CYP11A1, and via the acidic bile acid pathway, which is controlled by enzyme CYP27A1	Rattus norvegicus

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Release 2023.1 (January 2023)