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Literature summary for 1.14.15.15 extracted from
- Sterol 27-hydroxylase in bile acid biosynthesis. Mechanism of oxidation of 5beta-cholestane-3alpha,7alpha,12alpha,27-tetraol into 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid (1993), J. Biol. Chem., 266, 11079-11085.
No PubMed abstract available
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (25R)-5beta-cholestane-3alpha,7alpha,12alpha,27-tetraol + reduced adrenodoxin + O2 | Oryctolagus cuniculus | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidized adrenodoxin + H2O | - |
? |  |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | Oryctolagus cuniculus | - |
(25R)-5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | Oryctolagus cuniculus | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidzed adrenodoxin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + 6 reduced adrenodoxin + 6 H+ + 3 O2 = (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + 6 oxidized adrenodoxin + 4 H2O | reaction mechanism | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (25R)-5beta-cholestane-3alpha,7alpha,12alpha,27-tetraol + reduced adrenodoxin + O2 | - |
Oryctolagus cuniculus | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidized adrenodoxin + H2O | - |
? | |
| 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al + reduced adrenodoxin + O2 | - |
Oryctolagus cuniculus | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Oryctolagus cuniculus | (25R)-5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol + oxidized adrenodoxin + H2O | - |
? | |
| 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Oryctolagus cuniculus | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidzed adrenodoxin + H2O | - |
? | |
| additional information | the sterol 27-hydroxylase performs multiple monooxygenations in the conversion of 58-cholestane-3alpha,7alpha,12alpha-triol into 3alpha,7alpha,l2alpha-trihydroxy-5beta-cholestanoic acid, product identification by gas spectrometry | Oryctolagus cuniculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP27 | - |
Oryctolagus cuniculus |
| sterol 27-hydroxylase | - |
Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Oryctolagus cuniculus | |
| NADPH | - |
Oryctolagus cuniculus | |
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