Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1alpha-hydroxyvitamin D3 | competes with 5beta-cholestane-3alpha,7alpha,12alpha-triol | Rattus norvegicus | |
5beta-cholestane-3alpha,7alpha,12alpha-triol | competes with 1alpha-hydroxyvitamin D3 | Rattus norvegicus | |
N-bromosuccinimide | 50% loss of activity at 0.1 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00233 | - |
1alpha-hydroxyvitamin D3 as substrate | Rattus norvegicus |
0.114 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol as substrate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1alpha-hydroxyvitamin D3 + reduced adrenodoxin + O2 | 25-hydroxylation | Rattus norvegicus | 1alpha,25-dihydroxyvitamin D3 + oxidized adrenodoxin + H2O | - |
? | |
5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
5beta-cholestane-3alpha,7alpha-diol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
90% loss of activity after 2 min | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0233 | - |
1alpha-hydroxyvitamin D3 | - |
Rattus norvegicus | |
0.217 | - |
5beta-cholestane-3alpha,7alpha-diol | - |
Rattus norvegicus | |
0.6 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus |