Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | at a CO:O2 ratio of 0.75 hydroxylation reaction is undetectable | Rattus norvegicus | |
dicoumarol | 10% inhibition at 0.05 mM | Rattus norvegicus | |
p-chloromercuriphenyl sulfonate | 80% inhibition at 0.1 mM | Rattus norvegicus | |
Phenyl isocyanide | 70% inhibition at 0.1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 0.02 | O2 | - |
Rattus norvegicus | |
0.06 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | inner membrane-matrix | Rattus norvegicus | 31966 | - |
additional information | only the mitochondrial hydroxylase system is specific for C-26 position, the microsomal system is unspecific for C-26 position but rather more active for other adjacent positions | Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | isocitrate can act as electron donor | Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | mitochondrial enzyme is specific for 26-hydroxylation whereas the reaction with the microsomal enzyme hydroxylates at position 23, 24, 25 and 26 | Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | possible function of a cytochrome-P450-like entity in the intramitochondrial 26-hydroxylase system | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |