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Literature summary for 1.14.15.13 extracted from

  • Cryle, M.J.; Bell, S.G.; Schlichting, I.
    Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase (2010), Biochemistry, 49, 7282-7296.
    View publication on PubMed
Search for more literature for 1.14.15.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme A356T, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 0.1 M MgCl2, 12% (w/v) polyethylene glycol 3350 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
A356T mutant enzyme A356T crystallizes under several PEG-3350 conditions, with an optimum pH around 6 and a relatively low PEG concentration (12-15% (w/v)). The wild-type protein does not crystallize under these conditions, while the protein of the A356T mutant crystallizes readily and forms large, thin plates Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclo(L-leucyl-L-leucyl) + reduced ferredoxin + O2 Bacillus subtilis
-
 pulcherriminic acid + oxidized ferredoxin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34926
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Resource Q column chromatography, and Superose-12 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenylimidazole + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
2,5-di-tert-butylhydroquinone + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
2,5-di-tert-butylquinone + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
2-phenylimidazole + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
4-phenylimidazole + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
cyclo(L-alanyl-L-alanyl) + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
cyclo(L-leucyl-L-leucyl) + reduced ferredoxin + O2
-
 Bacillus subtilis pulcherriminic acid + oxidized ferredoxin + H2O
-
 ?
cyclo(L-leucyl-L-phenylalanyl) + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
cyclo(L-leucyl-L-prolyl) + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
cyclo(L-leucyl-L-tryptophanyl) + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
cyclo(L-methionyl-L-methionyl) + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
cyclo(L-valyl-L-valyl) + reduced ferredoxin + O2
-
 Bacillus subtilis ? + oxidized ferredoxin + H2O
-
 ?
additional information the enzyme does not use dimethylpyrazine and tetramethylpyrazine as substrates Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
cyclo-L-leucyl-L-leucyl dipeptide oxidase
-
 Bacillus subtilis
CYP134A1
-
 Bacillus subtilis
CypX
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Bacillus subtilis