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Literature summary for 1.14.15.1 extracted from

  • Dang, M.; Pochapsky, S.; Pochapsky, T.
    Spring-loading the active site of cytochrome P450 cam (2011), Metallomics, 3, 339-343.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.1

Protein Variants

Protein Variants Comment Organism
G326A site-directed mutagenesis in order to decrease the flexibility of the polypeptide at that point, spin state fractions with different substrates and compared to the wild-type enzyme. The mutant shows 40% reduced activity compared to the wild-type enzyme Pseudomonas putida
additional information generation of a G327 insertion mutant in order to determine whether the length of the helix played a role in the sensitivity of the K' helix to substrate, the insertion mutant fails to express Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme and cytochrome P450 containing enzyme Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture Pseudomonas putida strains can use (1R)-(+) camphor as sole carbon and energy source Pseudomonas putida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
adamantenone + reduced putidaredoxin + O2
-
 Pseudomonas putida ?
-
 ?
norcamphor + reduced putidaredoxin + O2
-
 Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP101A1
-
 Pseudomonas putida
cytochrome P450 cam
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Pseudomonas putida
heme
-
 Pseudomonas putida
putidaredoxin
-
 Pseudomonas putida

General Information

General Information Comment Organism
evolution the enzyme belongs to the superfamily of cytochrome P450 monooxygenases Pseudomonas putida
additional information substrate recognition and selectivity, enzyme-substrate interactions, NMR structrue analysis, Analysis of 1H,15N-TROSY-HSQC spectra of CYP-(+)-camphor-CO, CYP-adamantenone-CO and CYP-norcamphor-CO. overview. Replacing the native substrate camphor with adamantanone or norcamphor causes perturbations in NMR-detected NH correlations assigned to the network,which includes portions of a beta-sheet and an adjacent helix that is remote from the active site Pseudomonas putida