Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.1 extracted from

  • Prasad, B.; Rojubally, A.; Plettner, E.
    Identification of camphor oxidation and reduction products in Pseudomonas putida: new activity of the cytochrome P450cam system (2011), J. Chem. Ecol., 37, 657-667.
    View publication on PubMed
Search for more literature for 1.14.15.1

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme is CAM plasmid encoded Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains [2Fe–2 S] cluster and cytochrome P450 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida under low oxygen conditions borneol is formed instead of 5-ketocamphor borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida ATCC 17453
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida ATCC 17453 under low oxygen conditions borneol is formed instead of 5-ketocamphor borneol + oxidized putidaredoxin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ATCC 17453
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native C334A enzyme mutant from Pseudomonas putida strain ATCC 17453 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida ATCC 17453 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 under low oxygen conditions borneol is formed instead of 5-ketocamphor Pseudomonas putida borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 the hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen is coupled to the oxidation of the substrate. A key intermediate in the catalytic cycle is the iron-oxo species (Fe(IV)=O) Pseudomonas putida borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 under low oxygen conditions Pseudomonas putida borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 under low oxygen conditions borneol is formed instead of 5-ketocamphor Pseudomonas putida ATCC 17453 borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 the hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen is coupled to the oxidation of the substrate. A key intermediate in the catalytic cycle is the iron-oxo species (Fe(IV)=O) Pseudomonas putida ATCC 17453 borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-camphor + reduced putidaredoxin + O2 under low oxygen conditions Pseudomonas putida ATCC 17453 borneol + oxidized putidaredoxin + H2O
-
 ?
(+)-exo-5-hydroxycamphor + reduced putidaredoxin + O2
-
 Pseudomonas putida 5-oxocamphor + oxidized putidaredoxin + H2O
-
 ?
additional information under conditions of low oxygen, Pseudomonas putida cells and the isolated P450cam reduce camphor to borneol, product analysis by GC-MS Pseudomonas putida ?
-
 ?
additional information under conditions of low oxygen, Pseudomonas putida cells and the isolated P450cam reduce camphor to borneol, product analysis by GC-MS Pseudomonas putida ATCC 17453 ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP101
-
 Pseudomonas putida
cytochrome p450cam
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
heme CYP101 is a heme monoxygenase Pseudomonas putida
putidaredoxin
-
 Pseudomonas putida

General Information

General Information Comment Organism
additional information P450cam from Pseudomonas putida oxidizes (1R)-(+)-camphor to 5-exo-hydroxy camphor and further to 5-oxo-camphor Pseudomonas putida
physiological function Pseudomonas putida is capable of detoxification of camphor and borneol, overview Pseudomonas putida