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Literature summary for 1.14.15.1 extracted from

  • Davydov, R.; Perera, R.; Jin, S.; Yang, T.C.; Bryson, T.A.; Sono, M.; Dawson, J.H.; Hoffman, B.M.
    Substrate modulation of the properties and reactivity of the oxy-ferrous and hydroperoxo-ferric intermediates of cytochrome P450cam as shown by cryoreduction-EPR/ENDOR spectroscopy (2005), J. Am. Chem. Soc., 127, 1403-1413.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
T252A site-sirected mutagenesis, comparison of substrate binding properties to the wild-type enzyme, overview Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information EPR/ENDOR studies of wild-type and mutant T252A enzymes Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme iron, formation of an oxyferrous cytochrome P450cam during reaction, structure of the active site of oxyferrous-P450cam Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O via H2O2 and a nucleophilic intermediate, the substrate can modulate the properties of both the monoxygenase active-oxygen intermediates and the proton-delivery network that encompasses them, the catalytic cycle, overview Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin
-
Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
5,5-difluorocamphor + O2 + reduced putidaredoxin
-
Pseudomonas putida ?
-
?
5-methylenyl-camphor + O2 + reduced putidaredoxin
-
Pseudomonas putida ?
-
?
adamantanone + O2 + reduced putidaredoxin
-
Pseudomonas putida ?
-
?
additional information the properties and reactivity of the oxyheme and of both the primary and the annealed intermediates are modulated by a bound substrate, including alterations in the properties of the heme center, the presence of any alternative substrate increases the lifetime of hydroperoxoferri-P450cam no less than about 20fold, especially 5-methylenyl-camphor Pseudomonas putida ?
-
?
norcamphor + O2 + reduced putidaredoxin
-
Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
cytochrome p450cam
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
putidaredoxin
-
Pseudomonas putida