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Literature summary for 1.14.15.1 extracted from
- Effect of oxygen on the dehalogenation of 1,2-dibromo-3-chloropropane by cytochrome P450cam (CYP101) (2005), Biotechnol. Prog., 21, 1119-1127.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of enzyme and cofactor, separately, in Escherichia coli strain DH5alpha | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | development of a global data fitting kinetic model that describes the time-varying concentrations of substrate and products, Michaelis-Menten kinetics | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + O2 + reduced putidaredoxin | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme to 90% purity from Escherichia coli strain DH5alpha | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
| 1,2-dibromo-3-chloropropane + O2 + reduced putidaredoxin | dehalogenation, bromochloroacetone is the major conversion product when the incubation medium is saturated with oxygen, while allyl chloride is the sole product in the absence of oxygen | Pseudomonas putida | 1-bromo-3-chloroacetone + allyl chloride + H2O + putidaredoxin + Br- | a number of bromochloropropene are also formed to a minor extent by an elimination mechanism, product determination | ? | |
| additional information | relative stability of dibromochloropropane and products, overview | Pseudomonas putida | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP101 | - |
Pseudomonas putida |
| cytochrome p450cam | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| putidaredoxin | - |
Pseudomonas putida |
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