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Literature summary for 1.14.15.1 extracted from

  • Manna, S.K.; Mazumdar, S.
    Role of threonine 101 on the stability of the heme active site of cytochrome P450cam: multiwavelength circular dichroism studies (2006), Biochemistry, 45, 12715-12722.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene camC, expression of mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
T101V site-directed mutagenesis, the mutant shows decreased thermal stability of the heme active site and reaction intermediates in the reaction, equilibrium unfolding compared to the wild-type enzyme Pseudomonas putida

General Stability

General Stability Organism
Thr101 is required for the enzyme reaction intermediate Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermal unfolding kinetics, substrate binding kinetics Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme iron, located in the active site, Thr101 is important for stability, tertiary and secondary structure of the heme active site, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P00183 strain containing the C334A mutant enzyme, gene camC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin
-
Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?

Subunits

Subunits Comment Organism
More tertiary and secondary structure of the heme active site, overview Pseudomonas putida

Synonyms

Synonyms Comment Organism
cytochrome p450cam
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal unfolding data, thermal multistep unfolding modell, Thr101 is important for thermal stability, equilibrium unfolding of wild-type C334A and mutant enzymes Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
putidaredoxin
-
Pseudomonas putida