Application | Comment | Organism |
---|---|---|
synthesis | in vivo production of ortho-hydroxylated flavonoids by recombinant Escherichia coli. When HpaC is linked with an S-Tag on the C terminus, the enzyme activity is significantly affected. The optimal culture conditions are a substrate concentration of 80 mg/l, an induction temperature of 28°C, an M9 medium, and a substrate delay time of 6 h after IPTG induction. The efficiency of eriodictyol conversion from recombinant strains fed naringin is up to 57.67. Highest conversion efficiencies for production of catechin and caffeate are 35.2 % and 32.93%, respectively | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A0A140NG21 | - |
- |
Escherichia coli BL21-DE3 | A0A140NG21 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naringin + NADH + H+ + O2 | - |
Escherichia coli | eryodictyol + NADP+ + H2O | - |
? | |
naringin + NADH + H+ + O2 | - |
Escherichia coli BL21-DE3 | eryodictyol + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58500, SDS-PAGE, recombinant protein | Escherichia coli |