Cloned (Comment) | Organism |
---|---|
gene hpaH, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in enzyme-deficient Escherichia coli strain DH5alpha | Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56269, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Geobacillus sp. |
56269 | - |
x * 56269, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Geobacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + H+ + O2 | Geobacillus sp. | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + H+ + O2 | Geobacillus sp. PA-9 | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | Q4L1M7 | gene hpaH | - |
Geobacillus sp. PA-9 | Q4L1M7 | gene hpaH | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain DH5alpha by affinity chromatography on a 4-hydroxyphenylacetate-coupled amino-agarose column to near homogeneity | Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + H+ + O2 | - |
Geobacillus sp. | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + H+ + O2 | compared with intracellular cell extracts, the purified recombinant HpaH protein does not display detectable activity toward the 4-hydroxyphenylacetate substrate under the assay conditions used, in contrast to the cell extract, overview | Geobacillus sp. | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + H+ + O2 | - |
Geobacillus sp. PA-9 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + H+ + O2 | compared with intracellular cell extracts, the purified recombinant HpaH protein does not display detectable activity toward the 4-hydroxyphenylacetate substrate under the assay conditions used, in contrast to the cell extract, overview | Geobacillus sp. PA-9 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56269, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Geobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
4-HPA hydroxylase | - |
Geobacillus sp. |
HPAH | - |
Geobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Geobacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Geobacillus sp. |