Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.9 extracted from

  • Gibello, A.; Suarez, M.; Allende, J.L.; Martin, M.
    Molecular cloning and analysis of the genes encoding the 4-hydroxyphenylacetate hydroxylase from Klebsiella pneumoniae (1997), Arch. Microbiol., 167, 160-166.
    View publication on PubMed
Search for more literature for 1.14.14.9

Cloned(Commentary)

Cloned (Comment) Organism
HpaA and HpaH are expressed in Escherichia coli W-21, CC118 and YS1 as well as Klebsiella pneumonia mutant strain AG813 Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information mutant AG813, defective in the 4-hydroxyphenylacetate hydroxylase Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18680
-
 x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence Klebsiella pneumoniae
58781
-
 x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 Klebsiella pneumoniae meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 M5a1 strain UN
-

Purification (Commentary)

Purification (Comment) Organism
partially Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O two protein component enzyme: flavin-containing hydroxylase and coupling protein that appears to be an effector protein, both needed for productive hydroxylation. Enzyme synthesis is induced by 3- or 4-hydroxyphenylacetate Klebsiella pneumoniae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + ? 30% of 4-hydroxyphenylacetate activity Klebsiella pneumoniae ?
-
 ?
3,4-dihydroxyphenylacetate + ? 50% of 4-hydroxyphenylacetate activity Klebsiella pneumoniae ?
-
 ?
3-hydroxyphenylacetate + NADH + O2
-
 Klebsiella pneumoniae 3,4-hydroxyphenylacetate + NAD+ + H2O
-
 ?
4-hydroxybenzoate + ? 15% of 4-hydroxyphenylacetate activity Klebsiella pneumoniae 3,4-dihydroxybenzoate + ?
-
 ?
4-hydroxyphenylacetate + NADH + O2
-
 Klebsiella pneumoniae 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
4-hydroxyphenylacetate + NADH + O2 meta-cleavage pathway Klebsiella pneumoniae 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
catechol + ? 30% of 4-hydroxyphenylacetate activity Klebsiella pneumoniae ?
-
 ?
L-(3,4-dihydroxy)phenylalanine + ? 10% of 4-hydroxyphenylacetate Klebsiella pneumoniae ?
-
 ?
L-tyrosine + ? 8% of 4-hydroxyphenylacetate activity Klebsiella pneumoniae L-(3,4-dihydroxy)phenylalanine + ?
-
 ?
additional information dihydroxylated compounds are transformed, no reaction with phenylacetate, 4-chlorobenzoate or 2-hydroxyphenylacetate Klebsiella pneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
FAD stimulates Klebsiella pneumoniae
NADH
-
 Klebsiella pneumoniae