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Literature summary for 1.14.14.9 extracted from

  • Barbour, M.G.; Bayly, R.C.
    Control of meta-cleavage degradation of 4-hydroxyphenylacetate in Pseudomonas putida (1981), J. Bacteriol., 147, 844-850.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information several mutants are created, e.g. strains with enzyme defectives for analysis of the 4-hydroxyphenylacetate pathway Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida NCIB 9865 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
P23X1, NCIB 9865
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Pseudomonas putida NCIB 9865 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
4-hydroxyphenylacetate + NADH + O2 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway Pseudomonas putida NCIB 9865 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
?
additional information strict substrate specificity Pseudomonas putida ?
-
?
additional information strict substrate specificity Pseudomonas putida NCIB 9865 ?
-
?