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Literature summary for 1.14.14.43 extracted from

  • Naur, P.; Petersen, B.L.; Mikkelsen, M.D.; Bak, S.; Rasmussen, H.; Olsen, C.E.; Halkier, B.A.
    CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis (2003), Plant Physiol., 133, 63-72.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.43

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 indole-3-acetaldoxime pH 8.1, 29°C Arabidopsis thaliana
0.156
-
 (E)-4-hydroxyphenylacetaldoxime pH 8.1, 29°C Arabidopsis thaliana
0.556
-
 (E)-phenylacetaldoxime pH 8.1, 29°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P48421
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-hydroxyphenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 1-aci-nitro-2-(4-hydroxyphenyl)-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
(E)-phenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 1-aci-nitro-2-phenylethane + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
indole-3-acetaldoxime + O2 + glutathione + [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 1-aci-nitro-2-indolyl-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?

Synonyms

Synonyms Comment Organism
CYP83A1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
 (E)-phenylacetaldoxime pH 8.1, 29°C Arabidopsis thaliana
0.43
-
 (E)-4-hydroxyphenylacetaldoxime pH 8.1, 29°C Arabidopsis thaliana
2.33
-
 indole-3-acetaldoxime pH 8.1, 29°C Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function aliphatic oximes derived from chain-elongated homologs of methionine are efficiently metabolized by isoform CYP83A1, whereas CYP83B1 metabolizes these substrates with very low efficiency. Aromatic oximes derived from phenylalanine, tryptophan, and tyrosine are metabolized by both enzymes, although CYP83B1 has higher affinity for these substrates than CYP83A1 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.75
-
 (E)-phenylacetaldoxime pH 8.1, 29°C Arabidopsis thaliana
2.76
-
 (E)-4-hydroxyphenylacetaldoxime pH 8.1, 29°C Arabidopsis thaliana
15
-
 indole-3-acetaldoxime pH 8.1, 29°C Arabidopsis thaliana