Cloned (Comment) | Organism |
---|---|
expression in Nicotiana tabacum | Sorghum bicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sorghum bicolor | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-Hydroxyphenylacetaldehyde oxime | Sorghum bicolor | - |
(Z)-4-Hydroxyphenylacetaldehyde oxime | - |
? | |
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | Sorghum bicolor | - |
(S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
(Z)-4-hydroxyphenylacetaldehyde oxime | Sorghum bicolor | - |
4-hydroxyphenylacetonitrile + H2O | - |
? | |
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | Sorghum bicolor | - |
(S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | O48958 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-Hydroxyphenylacetaldehyde oxime | - |
Sorghum bicolor | (Z)-4-Hydroxyphenylacetaldehyde oxime | - |
? | |
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sorghum bicolor | (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O | overall reaction | ? | |
(Z)-4-hydroxyphenylacetaldehyde oxime | - |
Sorghum bicolor | 4-hydroxyphenylacetonitrile + H2O | - |
? | |
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sorghum bicolor | (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | the biosynthetic pathway for the cyanogenic glucoside dhurrin in Sorghum involves the sequential production of (E)- and (Z)-4-hydroxyphenylacetaldoxime. Monooxygenae CYP79A1 catalyzes conversion of tyrosine to (E)-4-hydroxyphenylacetaldoxime, whereas monooxygenase CYP71E1 catalyzes conversion of (E)-4-hydroxyphenylacetaldoxime into the corresponding geometrical Z-isomer as required for its dehydration into a nitrile, the next intermediate in cyanogenic glucoside synthesis | Sorghum bicolor |