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Literature summary for 1.14.14.33 extracted from

  • Payne, J.W.; Bolton, H.; Campbell, J.A.; Xun, L.
    Purification and characterization of EDTA monooxygenase from the EDTA-degrading bacterium BNC1 (1998), J. Bacteriol., 180, 3823-3827.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.33

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0085
-
 ethylenediaminetetraacetate presence of Mg2+, pH 7.8, 35°C EDTA-degrading bacterium BNC1
0.034
-
 ethylenediaminetetraacetate no addition of divalent ions, pH 7.8, 35°C EDTA-degrading bacterium BNC1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgEDTA2- is the best substrate EDTA-degrading bacterium BNC1
additional information the enzyme is able to release glyoxylate from EDTA complexed with any of the cations tested (Mg2+, Mn2+, Ni2+, Co2+, Zn2+, Fe2+, Ca2+, Cu2+, Cr2+, Sn2+, Ba2+, Cd2+, Sr2+, Pd2+, Al3+, Cr3+, K+, or Na+). MgEDTA2- is the best substrate EDTA-degrading bacterium BNC1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration EDTA-degrading bacterium BNC1

Organism

Organism UniProt Comment Textmining
EDTA-degrading bacterium BNC1 Q9F9T3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 EDTA-degrading bacterium BNC1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethylenetriaminepentaacetate + FMNH2 + O2
-
 EDTA-degrading bacterium BNC1 diethylenetriaminetetraacetate + glyoxylate + FMN + H2O
-
 ?
ethylenediaminetetraacetate + 2 FMNH2 + 2 O2
-
 EDTA-degrading bacterium BNC1 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O
-
 ?
additional information the enzyme oxidizes both EDTA complexed with various metal ions and uncomplexed EDTA EDTA-degrading bacterium BNC1 ?
-
 ?
nitrilotriacetate + FMNH2 + O2
-
 EDTA-degrading bacterium BNC1 nitrilodiacetate + glyoxylate + FMN + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE EDTA-degrading bacterium BNC1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 EDTA-degrading bacterium BNC1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25
-
 75% of maximum activity EDTA-degrading bacterium BNC1
40
-
 96% of maximum activity EDTA-degrading bacterium BNC1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 ethylenediaminetetraacetate no addition of divalent ions, pH 7.8, 35°C EDTA-degrading bacterium BNC1
3.6
-
 ethylenediaminetetraacetate presence of Mg2+, pH 7.8, 35°C EDTA-degrading bacterium BNC1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 EDTA-degrading bacterium BNC1

pH Range

pH Minimum pH Maximum Comment Organism
6.9
-
 75% of maximum activity EDTA-degrading bacterium BNC1
8.1
-
 70% of maximum activity EDTA-degrading bacterium BNC1

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
 EDTA-degrading bacterium BNC1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
80
-
 ethylenediaminetetraacetate no addition of divalent ions, pH 7.8, 35°C EDTA-degrading bacterium BNC1
430
-
 ethylenediaminetetraacetate presence of Mg2+, pH 7.8, 35°C EDTA-degrading bacterium BNC1