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Literature summary for 1.14.14.3 extracted from
- Activities, kinetics and emission spectra of bacterial luciferase-fluorescent protein fusion enzymes (2011), Photochem. Photobiol., 87, 1346-1353.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme) | Vibrio harveyi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00018 | - |
FMNH2 | luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi |  |
| 0.0002 | - |
FMNH2 | recombinant wild type enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
| 0.00113 | - |
decanal | recombinant wild type enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
| 0.00165 | - |
decanal | luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoic acid + FMN + H2O + hv | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| luciferase | - |
Vibrio harveyi |
| Lux | - |
Vibrio harveyi |
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