Cloned (Comment) | Organism |
---|---|
the bacterial luciferase lux gene cassette consists of five genes, luxCDABE. The lux operon is re-synthesized through a process of multibicistronic, codon-optimization to demonstrate self-directed bioluminescence emission in a mammalian HEK-293 cell line in vitro and in vivo, overview. To overcome the limitations by FMNH2 supply, co-expression of a constitutively expressed flavin reductase gene frp from Vibrio harveyi is performed leading to a 151fold increased increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | codon optimization of the luxCDE and frp genes, e.g. adaptation of the bacterial protein to mammalian temperature of 37°C, detailed overview | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + FMNH2 + O2 | Vibrio harveyi | - |
a carboxylate + FMN + H2O + hnu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + FMNH2 + O2 | - |
Vibrio harveyi | a carboxylate + FMN + H2O + hnu | - |
? | |
an aldehyde + FMNH2 + O2 | a long chain aliphatic aldehyde as substrate | Vibrio harveyi | a carboxylate + FMN + H2O + hnu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Vibrio harveyi |
General Information | Comment | Organism |
---|---|---|
physiological function | the luxCDABE encoded protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations | Vibrio harveyi |