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Literature summary for 1.14.14.3 extracted from

  • Campbell, Z.T.; Baldwin, T.O.
    Fre Is the Major Flavin Reductase Supporting Bioluminescence from Vibrio harveyi Luciferase in Escherichia coli (2009), J. Biol. Chem., 284, 8322-8328.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli from pJHD500, ligated into a pET21b vector. Luciferase subcloned from pZCH2 into a pASKIBA-3c vector with the restriction sites XbaI and XhoI. The resulting luciferase containing a strep-II tag on the C terminus of the beta-subunit (pZCB4) expressed Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 FMN in the presence of different flavin concentrations, 0.001 mM Fre oxidoreductase, 10 mM decanal, and 0.01 mM NADPH and 0.005 mM luciferase Vibrio harveyi
0.0013
-
 riboflavin in the presence of different flavin concentrations, 0.001 mM Fre oxidoreductase, 10 mM decanal, and 0.01 mM NADPH and 0.005 mM luciferase Vibrio harveyi

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Vibrio harveyi
Na+
-
 Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldehyde + FMNH2 + O2
-
 Vibrio harveyi ?
-
 ?
decanal + FMNH + O2
-
 Vibrio harveyi decanoic acid + FMN + H2O + light
-
 ?
decanal + riboflavin + O2 riboflavin is a very poor substrate for bacterial luciferase Vibrio harveyi ?
-
 ?

Synonyms

Synonyms Comment Organism
bacterial luciferase
-
 Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
FMNH
-
 Vibrio harveyi
FMNH2
-
 Vibrio harveyi

General Information

General Information Comment Organism
physiological function bacterial luciferase requires an exogenous source of reduced flavin mononucleotide for bioluminescence activity. There is no stable complex formation between luciferase and oxidoreductases Fre/NfsA from Escherichia coli or Frp from Vibrio harveyi, which are believed to provide reduced flavin for luciferase activity. No difference in the levels of luciferase expression in either the DELTAfre or DELTANsfA strains Vibrio harveyi