Cloned (Comment) | Organism |
---|---|
gene Gluc, expression in Mycobacterium smegmatis using an hsp60 promoter, subcloning in Escherichia coli strain DH5alpha | Gaussia princeps |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + FMNH2 + O2 | Gaussia princeps | an imidazolopyrazine derivative | CO2 + coelenteramide + FMN + light + H2O | - |
ir | |
additional information | Gaussia princeps | the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaussia princeps | - |
gene Gluc | - |
Oxidation Stability | Organism |
---|---|
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature | Gaussia princeps |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + FMNH2 + O2 | an imidazolopyrazine derivative | Gaussia princeps | CO2 + coelenteramide + FMN + light + H2O | - |
ir | |
additional information | the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature | Gaussia princeps | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gluc luciferase | - |
Gaussia princeps |
luciferase | - |
Gaussia princeps |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature | Gaussia princeps |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature | Gaussia princeps |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Gaussia princeps |