Activating Compound | Comment | Organism | Structure |
---|---|---|---|
estrogen | overexpression of PI3K or Akt significantly increases estrogenic upregulation of CYP7B1 in Hep-G2 cells. Estrogen receptor interacts with the PI3K/Akt pathway without binding to the CYP7B1 gene. In HEK293 cells, CYP7B1 transcription is much less affected by Akt, indicating that the mechanism for upregulation of CYP7B1 is different in different cell types | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LY294002 | specific inhibitor of phosphatidylinositol 3-kinase, abolishes estrogen receptor-mediated upregulation of a CYP7B1 promoter-luciferase reporter in Hep-G2 cells but not in HEK-293 cells | Homo sapiens | |
additional information | overexpression of Akt, in the absence of estrogen stimulation, suppresses basal CYP7B1 promoter activity by about 75%. Overexpression of dominant-negative mutant Akt abolishes estrogen receptor-mediated stimulation of CYP7B1 in Hep-G2 cells | Homo sapiens | |
PD98059 | markedly suppresses basal CYP7B1 promoter activity, abolishes upregulation of CYP7B1 by estrogen receptor in Hep-G2 cells | Homo sapiens | |
SP600125 | markedly suppresses basal CYP7B1 promoter activity, abolishes upregulation of CYP7B1 by estrogen receptor in Hep-G2 cells but not in HEK-293 cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroepiandrosterone + NADPH + H+ + O2 | - |
Homo sapiens | ? + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP7B1 | - |
Homo sapiens |
Steroid hydroxylase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | expression of CYP7B1 by estrogen receptor can be mediated via the PI3K/Akt signal pathway. Treatment with LY294002, a specific inhibitor of phosphatidylinositol 3-kinase PI3K, abolishes estrogen-receptor-mediated up-regulation of a CYP7B1 promoter-luciferase reporter | down |
Homo sapiens | expression of CYP7B1 by estrogen receptor can be mediated via the PI3K/Akt signal pathway. Overexpression of PI3K or Akt significantly increases estrogenic up-regulation of CYP7B1 | up |
General Information | Comment | Organism |
---|---|---|
physiological function | important role for CYP7B1 in cellular growth, particularly in connection with estrogenic signalling | Homo sapiens |