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Literature summary for 1.14.14.27 extracted from
- Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC (1976), Eur. J. Biochem., 61, 259-269.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Pseudomonas putida |
| 70000 | - |
1 * 70000, SDS-PAGE | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| resorcinol + FADH2 + O2 | Pseudomonas putida | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | hydroxyquinol + FAD + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida ORC | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| resorcinol hydroxylase accounts for about 3% of the total protein in Pseudomonas putida ORC after growth on resorcinol | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.6 | - |
pH 6.8, 30°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| orcinol + FADH2 + O2 | 90% of the hydroxylation activity as compared to resorcinol | Pseudomonas putida | ? + FAD + H2O | - |
? | |
| resorcinol + FADH2 + O2 | - |
Pseudomonas putida | hydroxyquinol + FAD + H2O | - |
? | |
| resorcinol + FADH2 + O2 | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | Pseudomonas putida | hydroxyquinol + FAD + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| graA | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FADH2 | contains approximately 1 mol of FAD for each polypeptide chain. The enzyme uses FADH2 as a substrate rather than a cofactor. FADH2 is provided by flavin reductase (NADH) | Pseudomonas putida | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | Pseudomonas putida |
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