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Literature summary for 1.14.14.24 extracted from
- Identification of a novel rat microsomal vitamin D3 25-hydroxylase (2004), J. Biol. Chem., 279, 22848-22856.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Emulgen 911 | strong reversible inhibition | Rattus norvegicus | |
| Triton X-100 | strong reversible inhibition | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
1alpha-hydroxyvitamin D3 | pH 7.7, 37°C | Rattus norvegicus | |
| 0.00079 | - |
vitamin D3 | pH 7.7, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P51590 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | low expression | Rattus norvegicus | - |
| kidney | low expression | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6400 | - |
pH 7.7, 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1alpha-hydroxyvitamin D3 + O2 + NADPH + H+ | - |
Rattus norvegicus | 1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O | - |
? | |
| additional information | no substrate: testosterone | Rattus norvegicus | ? | - |
? | |
| vitamin D3 + O2 + NADPH + H+ | - |
Rattus norvegicus | 25-hydroxyvitamin D3 + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000-60000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP2J3 | - |
Rattus norvegicus |
| Cytochrome P450 2J3 | - |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
vitamin D3 | pH 7.7, 37°C | Rattus norvegicus | |
| 22 | - |
1alpha-hydroxyvitamin D3 | pH 7.7, 37°C | Rattus norvegicus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Rattus norvegicus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Rattus norvegicus | CYP2J3 mRNA is is expressed at a level similar to that of CYP27A1 without marked sexual dimorphism | additional information |
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Release 2023.1 (January 2023)
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