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Literature summary for 1.14.14.23 extracted from

  • Frank, D.J.; Waddling, C.A.; La, M.; Ortiz de Montellano, P.R.
    Cytochrome P450 125A4, the third cholesterol C-26 hydroxylase from Mycobacterium smegmatis (2015), Biochemistry, 54, 6909-6916.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycolicibacterium smegmatis enzyme CYP125A4 also oxidizes cholesterol, although it has a much higher activity for the oxidation of 7alpha-hydroxycholesterol, EC 1.14.14.23. The enzyme forms 7alpha,26-dihydroxycholesterol ?
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Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
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gene CYP125A4
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Source Tissue

Source Tissue Comment Organism Textmining
culture condition:cholesterol-grown cell Mycobacterium smegmatis can grow on cholesterol as the sole carbon source Mycolicibacterium smegmatis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme CYP125A4 also oxidizes cholesterol, although it has a much higher activity for the oxidation of 7alpha-hydroxycholesterol, EC 1.14.14.23. The enzyme forms 7alpha,26-dihydroxycholesterol Mycolicibacterium smegmatis ?
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?

Synonyms

Synonyms Comment Organism
CYP125A4
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Mycolicibacterium smegmatis
cytochrome P450 125A4
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Mycolicibacterium smegmatis
More cf. EC 1.14.15.15 Mycolicibacterium smegmatis

General Information

General Information Comment Organism
additional information the ability of CYP125A4 to oxidize 7alpha-hydroxycholesterol is due, at least in part, to the presence of a smaller amino acid side chain facing C-7 of the sterol substrate than in CYP125A3 Mycolicibacterium smegmatis
physiological function utilization of cholesterol is initiated by three cholesterol hydroxylases, CYP125A3, CYP142A2, and CYP125A4. A CYP125A3/CYP142A2 double knockout mutant of Mycobacterium smegmatis is still able to grow on cholesterol as sole carbom source, albeit at a slower rate than the wild-type Mycolicibacterium smegmatis