Application | Comment | Organism |
---|---|---|
medicine | in inborn error of metabolism known as Smith-Lemli-Opitz syndrome, 7-oxocholesterol levels may also be elevated in tissue and fluids as a result of CYP7A1 oxidation of 7-dehydrocholesterol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
7-dehydrocholesterol | pH 7.4, 30°C | Homo sapiens | |
0.0018 | - |
lathosterol | 7-oxocholestanol formation, pH 7.4, 30°C | Homo sapiens | |
0.0021 | - |
lathosterol | epoxide formation, pH 7.4, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-dehydrocholesterol + NADPH + H+ | Homo sapiens | - |
7-oxocholesterol + NADP+ + H2O | minor product: 7alpha,8alpha-epoxide | ? | |
lathosterol + NADPH + H+ | Homo sapiens | i.e. DELTA-dihydro-7-dehydrocholesterol | 7-oxocholestanol + cholestanol-7alpha,8alpha-epoxide + NADP+ + H2O | about 1:2 ratio of 7-oxo and epoxide products, the epoxide does not rearrange to the ketone | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-dehydrocholesterol + NADPH + H+ | - |
Homo sapiens | 7-oxocholesterol + NADP+ + H2O | minor product: 7alpha,8alpha-epoxide | ? | |
7-dehydrocholesterol + NADPH + H+ | - |
Homo sapiens | 7-oxocholesterol + NADP+ + H2O | reaction with 7-[2H1]dehydrocholesterol yields complete migration of deuterium in the product 7-oxocholesterol. Minor product: 7alpha8alpha-epoxide | ? | |
lathosterol + NADPH + H+ | i.e. DELTA-dihydro-7-dehydrocholesterol | Homo sapiens | 7-oxocholestanol + cholestanol-7alpha,8alpha-epoxide + NADP+ + H2O | about 1:2 ratio of 7-oxo and epoxide products, the epoxide does not rearrange to the ketone | ? |
Synonyms | Comment | Organism |
---|---|---|
CYP7A1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
7-dehydrocholesterol | pH 7.4, 30°C | Homo sapiens | |
0.06 | - |
lathosterol | 7-oxocholestanol formation, pH 7.4, 30°C | Homo sapiens | |
0.12 | - |
lathosterol | epoxide formation, pH 7.4, 30°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
7-dehydrocholesterol | pH 7.4, 30°C | Homo sapiens | |
30 | - |
lathosterol | 7-oxocholestanol formation, pH 7.4, 30°C | Homo sapiens | |
50 | - |
lathosterol | epoxide formation, pH 7.4, 30°C | Homo sapiens |