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Literature summary for 1.14.14.22 extracted from
- Dibenzothiophene desulfurizing enzymes from moderately thermophilic bacterium Bacillus subtilis WU-S2B: purification, characterization and overexpression (2005), J. Biosci. Bioeng., 100, 266-273.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bdsA, recombinant overexpression in Escherichia coli, coexpression with chaperone groEL/groES genes | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Bacillus subtilis |  |
| 4-chloromercuribenzoate | - |
Bacillus subtilis | |
| Ag+ | - |
Bacillus subtilis | |
| biphenyl | - |
Bacillus subtilis | |
| Co2+ | - |
Bacillus subtilis | |
| Cu2+ | - |
Bacillus subtilis | |
| EDTA | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| Ni2+ | - |
Bacillus subtilis | |
| Zn2+ | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, SDS-PAGE | Bacillus subtilis |
| 174000 | - |
recombinant enzyme, gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Bacillus subtilis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
gene bdsA | - |
| Bacillus subtilis WU-S2B | - |
gene bdsA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 82.6fold from strain WU-S2B by three different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography, recombinant enzyme from Escherichia coli 17.0fold to homogeneity by anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 855 | - |
purified native enzyme, pH 7.0, 50°C | Bacillus subtilis |
| 875 | - |
purified recombinant enzyme, pH 7.0, 50°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Bacillus subtilis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| additional information | coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2 | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 45000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BdsA | - |
Bacillus subtilis |
| DBT sulfone monooxygenase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, 30 min, 30% activity remaning | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | purified enzyme, stable at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Bacillus subtilis | |
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