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Literature summary for 1.14.14.22 extracted from
- Purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization (2000), Bioseparation, 9, 185-188.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | flavin reductase (DszD) is essential for the enzyme activity of DszA | Rhodococcus erythropolis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
| 97000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |  |
| dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis D-1 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | Q6WE15 | gene dszA | - |
| Rhodococcus erythropolis D-1 | Q6WE15 | gene dszA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 102fold to homogeneity from strain D-1 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 807 | - |
purified enzyme, pH 7.0, 35°C | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszA | Rhodococcus erythropolis | ? | - |
? | |
| additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszA | Rhodococcus erythropolis D-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
| dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rhodococcus erythropolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Rhodococcus erythropolis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | flavin reductase (DszD) is essential for the enzyme activity of DszA | Rhodococcus erythropolis |
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