Application | Comment | Organism |
---|---|---|
synthesis | phenol hydroxylase gene cloning from the thermophilic bacteria Geobacillus thermoglucosidasius is used to develop an effective method to convert tyrosol into the high-added-value compound hydroxytyrosol by hydroxylation | Parageobacillus thermoglucosidasius |
Cloned (Comment) | Organism |
---|---|
gene pheA1, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, cloning of the pheA1 promoter from Geobacillus thermoglucosidasius and use for enzyme expression in Escherichia coli strain Rosetta, tandem expression of genes pheA1 and pheA2 | Parageobacillus thermoglucosidasius |
gene pheA2, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain Rosetta tandem expression of genes pheA1 and pheA2 | Parageobacillus thermoglucosidasius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | optimisation of conditions for diphenol and hydroxytyrosol production by transformed Escherichia coli cells | Parageobacillus thermoglucosidasius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17800 | - |
2 * 17800, PheA2, about, sequence calculation | Parageobacillus thermoglucosidasius |
57000 | - |
2 * 57000, SDS-PAGE | Parageobacillus thermoglucosidasius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + FADH2 + O2 | Parageobacillus thermoglucosidasius | - |
catechol + FAD + H2O | - |
? | |
phenol + FADH2 + O2 | Parageobacillus thermoglucosidasius A7 | - |
catechol + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | Q9LAG2 | PheA2; gene pheA2 | - |
Parageobacillus thermoglucosidasius | Q9LAG3 | PheA1; gene pheA1 | - |
Parageobacillus thermoglucosidasius A7 | Q9LAG2 | PheA2; gene pheA2 | - |
Parageobacillus thermoglucosidasius A7 | Q9LAG3 | PheA1; gene pheA1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + FADH2 + O2 | - |
Parageobacillus thermoglucosidasius | catechol + FAD + H2O | - |
? | |
phenol + FADH2 + O2 | - |
Parageobacillus thermoglucosidasius A7 | catechol + FAD + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57000, SDS-PAGE | Parageobacillus thermoglucosidasius |
homodimer | 2 * 17800, PheA2, about, sequence calculation | Parageobacillus thermoglucosidasius |
More | the large subunit of this enzyme is encoded by pheA1 gene and gives rise to a 57 kDa protein. The protein has hydroxylase activity, is a homodimer, but has no activity by its own | Parageobacillus thermoglucosidasius |
More | the minor component encoded by pheA2 gene has a predicted molecular mass of 17.8 kDa, it is a homodimer and shows a specific NADH:FAD reductase activity | Parageobacillus thermoglucosidasius |
Synonyms | Comment | Organism |
---|---|---|
PheA1 | - |
Parageobacillus thermoglucosidasius |
PheA2 | - |
Parageobacillus thermoglucosidasius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Parageobacillus thermoglucosidasius |
General Information | Comment | Organism |
---|---|---|
physiological function | phenol hydroxylase is a two-component enzyme encoded by pheA1 and pheA2 genes and strictly dependent on NADH and FAD. The intracellular enzyme is a clear example of a two-component tetrameric flavoprotein hydroxylase, in which flavin reduction and substrate oxygenation take place in the same protein | Parageobacillus thermoglucosidasius |