Literature summary for 1.14.14.19 extracted from

Petrunak, E.M.; DeVore, N.M.; Porubsky, P.R.; Scott, E.E.
Structures of human steroidogenic cytochrome P450 17A1 with substrates (2014), J. Biol. Chem., 289, 32952-32964.

Search for more literature for 1.14.14.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme A105L in complex with progesterone, pregnenolone, 17alpha-hydroxypregnenolone, or abiraterone is crystallized by the hanging drop vapor diffusion method using 100 mM Tris-HCl, pH 8.5, 25% (w/v) PEG 4000, 150 mM magnesium chloride hexahydrate, and 4-6% (v/v) glycerol. Mutant enzyme A105L in complex with 17alpha-hydroxyprogesterone is crystallized by the hanging drop vapor diffusion method using 175 mM Tris-HCl, pH 8.5, 30% (w/v) PEG 3350, 250 mM lithium sulfate, and 3% (v/v) glycerol	Homo sapiens

Crystallization (Comment)

Organism

mutant enzyme A105L in complex with progesterone, pregnenolone, 17alpha-hydroxypregnenolone, or abiraterone is crystallized by the hanging drop vapor diffusion method using 100 mM Tris-HCl, pH 8.5, 25% (w/v) PEG 4000, 150 mM magnesium chloride hexahydrate, and 4-6% (v/v) glycerol. Mutant enzyme A105L in complex with 17alpha-hydroxyprogesterone is crystallized by the hanging drop vapor diffusion method using 175 mM Tris-HCl, pH 8.5, 30% (w/v) PEG 3350, 250 mM lithium sulfate, and 3% (v/v) glycerol

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A105L	the effect of the A105L mutation is to increase progesterone affinity at least 2fold but the mutant has little effect on the already lower affinity for either progesterone hydroxylation product	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
abiraterone	-	Homo sapiens
TOK-001	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.00038	-	pregnenolone	mutant enzyme A105L, at pH 7.4 and 37°C	Homo sapiens
0.00093	-	pregnenolone	wild type enzyme, at pH 7.4 and 37°C	Homo sapiens
0.00196	-	progesterone	mutant enzyme A105L, at pH 7.4 and 37°C	Homo sapiens
0.0105	-	progesterone	wild type enzyme, at pH 7.4 and 37°C	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
a C21-steroid + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	a 17alpha-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
additional information	Homo sapiens	a minor conformation may yield the minor 16alpha-hydroxyprogesterone metabolite from progesterone	?	-	?
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
progesterone + [reduced NADPH-hemoprotein reductase] + O2	Homo sapiens	-	17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P05093	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-nitrilotriacetic acid-agarose resin column chromatography, carboxymethyl-Sepharose column chromatography, and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
a C21-steroid + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	a 17alpha-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
additional information	a minor conformation may yield the minor 16alpha-hydroxyprogesterone metabolite from progesterone	Homo sapiens	?	-	?
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
progesterone + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP17A1	-	Homo sapiens
steroidogenic cytochrome P450 17A1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.0065	-	pregnenolone	wild type enzyme, at pH 7.4 and 37°C	Homo sapiens
0.0065	-	pregnenolone	mutant enzyme A105L, at pH 7.4 and 37°C	Homo sapiens
0.017	-	progesterone	wild type enzyme, at pH 7.4 and 37°C	Homo sapiens
0.019	-	progesterone	mutant enzyme A105L, at pH 7.4 and 37°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Homo sapiens