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Literature summary for 1.14.14.19 extracted from
- Phosphorylation of human cytochrome P450c17 by p38alpha selectively increases 17,20 lyase activity and androgen biosynthesis (2013), J. Biol. Chem., 288, 23903-23913.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109, co-expression of P450c17 and p38 in COS-1 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S427A | site-directed mutagenesis | Homo sapiens |
| S427D | site-directed mutagenesis | Homo sapiens |
| S427E | site-directed mutagenesis | Homo sapiens |
| T341A | site-directed mutagenesis | Homo sapiens |
| T341A/S427A | site-directed mutagenesis | Homo sapiens |
| T341D | site-directed mutagenesis | Homo sapiens |
| T341E | site-directed mutagenesis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | pyridinyl imidazole drugs SB202190 and SB203580 inhibit 17,20 lyase but not 17alpha-hydroxylase activity in human adrenocortical HCI-H295A cells | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P05093 | gene CYP17A1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | Ser/Thr phosphorylation of P450c17 appears to promote P450 oxidoreductase-P450c17 interaction. The kinase that phosphorylates P450c17 is p38alpha, the p38beta isozyme is inactive | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant membrane-embedded His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal cortex | - |
Homo sapiens | - |
| NCI-H295A cell | - |
Homo sapiens | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome P450c17 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | cytochrome P450c17 is a steroidogenic enzyme that catalyzes the steroid 17alpha-hydroxylation needed for glucocorticoid synthesis | Homo sapiens |
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Release 2023.1 (January 2023)
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