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Literature summary for 1.14.14.19 extracted from

  • Hough, D.; Cloete, S.W.; Storbeck, K.; Swart, A.C.; Swart, P.
    Cortisol production in sheep is influenced by the functional expression of two cytochrome P450 17alpha-hydroxylase/17,20-lyase (CYP17) isoforms (2013), J. Anim. Sci., 91, 1193-1206.
    View publication on PubMed
Search for more literature for 1.14.14.19

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, CYP17 genotyping, realtime PCR expression analysis Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 progesterone isozyme 1, pH 7.4, temperature not specified in the publication Ovis aries
0.0017
-
 pregnenolone isozyme 1, pH 7.4, temperature not specified in the publication Ovis aries
0.0018
-
 progesterone isozyme 2, pH 7.4, temperature not specified in the publication Ovis aries
0.0019
-
 pregnenolone isozyme 2, pH 7.4, temperature not specified in the publication Ovis aries

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Ovis aries
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ovis aries the bifunctional enzyme catalyzes two types of reactions, namely, a 17alpha-hydroxylation reaction and a 17,20-lyase reaction ?
-
 ?
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 Ovis aries
-
 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2 Ovis aries
-
 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries Q29497 GenBank accession numbers L40335 and AF251388 encodíng isozymes 1 and 2; two isozymes of cytochrome P450 17alpha-hydroxylase/17,20-lyase, that differ in two nucleotides, resulting in variants Ser210Gly and Tyr464Asn
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme catalyzes two types of reactions, namely, a 17alpha-hydroxylation reaction and a 17,20-lyase reaction Ovis aries ?
-
 ?
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2
-
 Ovis aries 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Ovis aries 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP17
-
 Ovis aries
cytochrome P450 17alpha-hydroxylase/17,20-lyase
-
 Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Ovis aries