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Literature summary for 1.14.14.18 extracted from

  • Jin, Q.; Yuan, X.; Cui, W.; Han, B.; Feng, J.; Xu, S.; Shen, W.
    Isolation and characterization of a heme oxygenase-1 gene from Chinese cabbage (2012), Mol. Biotechnol., 50, 8-17.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, functional expression of His-tagged HO1 in Escherichia coli Brassica rapa subsp. pekinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Brassica rapa subsp. pekinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Brassica rapa subsp. pekinensis 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heme + 3 AH2 + 3 O2 Brassica rapa subsp. pekinensis
-
biliverdin IXalpha + Fe2+ + CO + 3 A + 3 H2O
-
?

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. pekinensis H8XZ68 two types of isoforms: an inducible heme oxygenase-1, HO1, and a constitutive heme oxygenase-2/3, HO2/3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged HO1 from Escherichia coli by nickel affinity chromatography Brassica rapa subsp. pekinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf young Brassica rapa subsp. pekinensis
-
root
-
Brassica rapa subsp. pekinensis
-
seed germinating Brassica rapa subsp. pekinensis
-
seedling
-
Brassica rapa subsp. pekinensis
-
stem
-
Brassica rapa subsp. pekinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heme + 3 AH2 + 3 O2
-
Brassica rapa subsp. pekinensis biliverdin IXalpha + Fe2+ + CO + 3 A + 3 H2O
-
?

Subunits

Subunits Comment Organism
More three-dimensional structure of BrHO1, analysis and comparison, overview Brassica rapa subsp. pekinensis

Synonyms

Synonyms Comment Organism
heme oxygenase-1
-
Brassica rapa subsp. pekinensis
Ho1
-
Brassica rapa subsp. pekinensis

Expression

Organism Comment Expression
Brassica rapa subsp. pekinensis HO1 is induced by several stress conditions, e.g. osmotic and salinity stresses, cadmium exposure, H2O2, and hemin treatments up

General Information

General Information Comment Organism
physiological function BrHO1 may play an important role in abiotic stress tolerance of Chinese cabbage. Heme oxygenase is a regulatory enzyme that cleaves heme to biliverdin IXalpha, with the concomitant release of carbon monoxide and the production of free Fe2+ Brassica rapa subsp. pekinensis