Literature summary for 1.14.14.18 extracted from

Lai, W.; Chen, H.; Matsui, T.; Omori, K.; Unno, M.; Ikeda-Saito, M.; Shaik, S.
Enzymatic ring-opening mechanism of verdoheme by the heme oxygenase: a combined X-ray crystallography and QM/MM study (2010), J. Am. Chem. Soc., 132, 12960-12970.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
azide-bound Fe(II)-verdoheme-HmuO, 30°C, a hanging drop vapor diffusion method, the reservoir solution containing 50 mM MES, pH 5.4, 2.3-2.5 M ammonium sulfate, 0.27 M sodium bromide, and 0.1% dioxane, X-ray diffraction structure determination and analysis at 3.0 A resolution	Corynebacterium diphtheriae

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium diphtheriae	Q54AI1	-	-

Purification (Commentary)

Purification (Comment)	Organism
verdoheme-HmuO complex by anion exchange chromatography and gel filtration	Corynebacterium diphtheriae

Reaction

Reaction	Comment	Organism	Reaction ID
protoheme + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = biliverdin + Fe2+ + CO + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O	a three-step mechanism, mechanism of the the third step, heme oxygenase enzymatic ring-opening mechanism of verdoheme, a process which maintains iron homeostasis, overview. Reaction mechanism for the FeOOH pathway and the FeHOOH pathway, and complete triplet-state mechanism, overview	Corynebacterium diphtheriae	a

Synonyms

Synonyms	Comment	Organism
HmuO	-	Corynebacterium diphtheriae

General Information

General Information	Comment	Organism
additional information	QM/MM calculations based on this crystal structure exploring the reaction mechanisms starting from the FeOOH-verdoheme and FeHOOH-verdoheme complexes, which mimic, respectively, the O2- and H2O2-supported degradations. The rate-determining step is the initial O-O bond breaking step, which is either homolytic, for FeHOOH-verdoheme, or coupled to electron and proton transfers, in FeOOH-verdoheme. The FeHOOH-verdoheme complex is more reactive than the FeOOH-verdoheme complex	Corynebacterium diphtheriae

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Release 2023.1 (January 2023)