Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | 0.1 mM, slight stimulation, inhibition at 1 mM | Rattus norvegicus |
General Stability | Organism |
---|---|
50% loss of activity during one cycle of freezing and thawing | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1 mM, 81% inhibition | Rattus norvegicus | |
4-hydroxymercuribenzoate | 1 mM, complete inhibition | Rattus norvegicus | |
cysteine | 1 mM, 67% inhibition | Rattus norvegicus | |
dithiothreitol | 0.01 mM, 88% inhibition | Rattus norvegicus | |
Hg2+ | 0.3 mM, complete inhibition | Rattus norvegicus | |
iodoacetamide | 5 mM, 39% inhibition, 10 mM, 79% inhibition | Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
heme | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, possibly a trimer, SDS-PAGE | Rattus norvegicus |
180000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + 2 NADPH + 2 H+ + 2 O2 | Rattus norvegicus | involved in heme metabolism | biliverdin + Fe2+ + CO + 2 NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, Sephadex G-200 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.7 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 2 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + 2 NADPH + 2 H+ + 2 O2 | involved in heme metabolism | Rattus norvegicus | biliverdin + Fe2+ + CO + 2 NADP+ + H2O | - |
? | |
heme + electron donor + O2 | electron donor NADH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
heme + electron donor + O2 | NADPH is more effective than NADH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
heme + electron donor + O2 | electron donor NADPH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
methemoglobin + electron donor + O2 | 30% of activity with heme | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, possibly a trimer, SDS-PAGE | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at 0°C | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is more effective than NADH | Rattus norvegicus | |
NADPH | NADPH is more effective than NADH | Rattus norvegicus |