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Literature summary for 1.14.14.17 extracted from

  • Nagumo, A.; Kamei, T.; Sakakibara, J.; Ono, T.
    Purification and characterization of recombinant squalene epoxidase (1995), J. Lipid Res., 36, 1489-1497.
    View publication on PubMed
Search for more literature for 1.14.14.17

Activating Compound

Activating Compound Comment Organism Structure
additional information 105000 g supernatant, 8.5fold activation of microsomal enzyme, 3fold activation of N-terminal truncated recombinant enzyme Rattus norvegicus
NADPH cytochrome c reductase required for activity of N-terminal truncated recombinant enzyme in a concentration-dependent manner Rattus norvegicus
Triton X-100 0.1%, 36fold activation of microsomal enzyme, 0.05%, 34fold activation of N-terminal truncated recombinant enzyme Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of cDNA in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
NB-598 0.0000032 mM, 50% inhibition of microsomal enzyme, 0.0000019 mM, 50% inhibition of N-terminal truncated recombinant enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 squalene N-terminal truncated recombinant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, Ni-NTA-agarose, Blue Sepharose CL-6B Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 N-terminal truncated recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-oxidosqualene + NADPH + O2 N-terminal truncated recombinant enzyme Rattus norvegicus 2,3,22,23-dioxidosqualene + NADP+ + H2O
-
 ?
squalene + NAD(P)H + O2
-
 Rattus norvegicus (S)-squalene-2,3-epoxide + NADP+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 microsomal and N-terminal truncated recombinant enzyme Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD required for activity Rattus norvegicus
additional information neither FMN nor riboflavin can replace FAD Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000041
-
 NB-598 N-terminal truncated recombinant enzyme Rattus norvegicus